The present study was aimed to check the physiological and biochemical influence of NaCl stress on the calli of tissue culture responsive variety CP 77/400 in vitro. Four level of NaCl stress i.e. 50mM, 100mM, 150mM and 200mM was used in the experiment against the Control. The calli was exposed for 21 days (3 weeks) to different level of salinity and significant effects (P≤ 0.05) on the physiological and biochemical properties of the cells were observed. Relative growth rate was significantly affected and was 0.28 g week-1 at 200mM concentration as compared to other NaCl levels. Water content decreased as the level of salinity stress increased. At 200mM concentration water content was the lowest (66.000%) of all concentrations used. Electric Conductivity (EC) was higher at 200mM concentration (133.06 dSm-1 ). Calli cells respond to NaCl stress by increasing the accumulation of proline and sugar content and both were statistically significant (P≤ 0.05). Proline accumulation was maximum at 200mM (36.748 µM g-1 FW) concentration in comparison to control. Similarly sugar accumulation increased significantly at 200mM NaCl Concentration (1.69481 µM g-1 FW).
Saba Gul, Mohammad Sayyar Khan, Mazhar Ullah, Waqar Ahmad, Syed Usman Ali Shah, Memrez Khushal. (2017) PHYSIOLOGICAL AND BIOCHEMICAL ASSESSMENT OF SUGARCANE CALLI (CP 77/400) AGAINST VARIOUS LEVEL OF SALINITY STRESS, , Volume 14, Issue 2.
