Abstract
Teucrium oliverianum Ging ex Benth. is used in Mediterranean region as antidiabetic, analgesic and anti-inflammatory remedy. Literature search revealed that much work has been done on other species of genus Teucrium but very little research work is carried out on T. oliverianum. We screened it for phyto-constituents and antimicrobial activity. Seventy gram crude extract was prepared from whole plant using classical method and fractionated with organic solvents (n-hexane, chloroform, ethyl acetate and n-butanol) yielding 22g, 17g, 11g and 20g of each fraction respectively. Phytochemical analysis was done on the fractions using standard method. Antibacterial activity was investigated using agar well diffusion procedure against some pathogenic gram positive, gram negative bacterial and fungal strain Candida albicans. Phytochemical studies showed positive results for alkaloids, carbohydrates/glycosides, tannins and triterpenes/sterols and absence of Anthraquinones in all fractions. Saponins were present in hexane and ethyl acetate fractions while flavonoids were absent in n-butanol fraction only. Mild significant antibacterial activity was found in Chloroform fraction. Ethyl acetate and n-Butanolsamples exhibited high activity against E.coli. Antifungal activity was found in Hexane and Ethyl acetate fractions.HPLC fingerprinting of Teucrium oliverianum ethanol extract was performed which showed small peaks starting from 17thminute and major peaks at 31st and 32nd minute which can be used for identification of this plant.Thus in short, the study revealed that different fractions contain a number of active phyto-constituents and mild significant antibacterial and antifungal activity which may be useful for further exploration.
