Cassava is an important tropical starchy root crop which is also used to acquire biodiesel. The aim of the present study was to develop an easy high frequency reproducible method for plant propagation and regeneration through plant tissue culture of cassava (Manihot esculanta). Explant was initiated using Murashige and Skoog (Ms) with 3mg/L BAP and 1mg/L IBA. Adenine sulphate and glutamine were also added at the rate of 25 mg/L and 50 mg/L respectively, along with 0.6 g/L charcoal. Different concentrations (0.5-3 mg/L) of 6-Banzyl amino purine (BAP) and (0.025 and 0.5mg/L) of α-naphthaleneacetic acid (NAA) were applied for shoot elongation and multiplication. Most effective response of shoot length was obtained on medium having 1.5 mg/L BAP along with 0.025 mg/L NAA. Highly proficient root length was observed on MS in combination with 0.5 mg/L NAA.
