Abstract
Optimization parameters in submerged fermentation were studied for the production of α-amylase from Mucor geophillus IBGE 05 using agricultural wastes (sunflower waste, cotton stalk and rice husk) as well as agro industrial wastes (date syrup and molasses) as sources of carbon. Optimal conditions for the production of α-amylase (4.87 U/mL) by Mucor geophillus IBGE 05 were observed when the strain was grown on culture medium M1 containing yeast extract as a source of nitrogen, molasses as a source of carbon after 48 h of incubation at 35° C, initial pH 6.5, inoculum size of 5x106 conidia in 50 mL of culture medium and agitation rate of 150 rpm. The strain was thermostable (up to 60° C) and pH (up to 9.0) so it has a potential to be used in industries for enzyme production.
