Abstract
This study was conducted to investigate the influence of extraction conditions i.e.
water:fronds ratio (0.5:1 – 12:1), temperature (25 – 90˚C) and pH (3 – 11) on extraction
yield, crude protein content and emulsifying capacity of mucilage from Talinum
paniculatum fronds. Response surface methodology with a face cantered-central
composite design was applied to optimize the extraction conditions. With 20
experimental runs, extraction yield, crude protein content and emulsifying capacity of
the mucilage were recorded to be 2.32 – 4.90%, 15.05 – 30.97% and 8.05 – 37.93%,
respectively. Response surface analyses showed that increases in mucilage yield were
mainly due to significant (p < 0.05) quadratic effect of pH and also synergistic effect
between water:fronds ratio and pH. In contrast, significant (p < 0.05) quadratic effect
of temperature and its synergistic effect with water:fronds ratio led to increase in
emulsifying capacity of the mucilage. Furthermore, linear effect of pH seemed to
significantly (p < 0.05) increase the crude protein content, in addition to significant (p
< 0.05) synergistic effect between water:fronds ratio and pH. Experimental data for
each response were best fitted with a quadratic model, having high coefficients of
determination (R2 = 0.81 – 0.98) and no lack-of-fit. The optimum conditions for
mucilage extraction from T. paniculatum were obtained at water:fronds ratio of 8.4:1,
temperature of 90 ⁰C and pH of 8, providing 3.44 % yield, 29.35 % crude protein
content and 34.00 % emulsifying capacity of T. paniculatum mucilage.
Nor Hayati, Tengku Nur, Yusnita Hamzah. (2019) Optimization of extraction conditions on yield, crude protein content and emulsifying capacity of mucilage from Talinum paniculatum, Asian Journal of Agriculture and Biology, Volume 7, Issue 1.
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