Abstract
In this study, optimization of enzymatic protein hydrolysis conditions of flower crab
meat (FCM) to yield maximum angiotensin converting enzyme (ACE) inhibitory
activity was carried out. First, screening of commercial food grade enzymes
(Alcalase®, Neutrase®, Protamex® and papain) was carried out to select the most
suitable proteinase to yield ACE inhibitory activity. A 3-level face-centered central
composite design (CCD) was employed to optimize four hydrolysis conditions
including temperature (45-55°C), hydrolysis time (1-3 hr), pH (6-8) and enzyme to
substrate ratio (E/S) (1-3%). Half maximal inhibitory concentration (IC50) of FCM
hydrolysate prepared at optimum condition was also determined. It was found that the
highest ACE inhibitory activity (85.52%) was given by Neutrase® after 2 hr hydrolysis.
Hence, Neutrase® was used in the optimization study. It was found that the enzymatic
hydrolysis condition of FCM towards ACE inhibitory activity could be predicted by a
quadratic model. The optimum enzymatic hydrolysis condition to obtain maximum
ACE inhibitory activity was at temperature of 54°C, E/S of 3%, pH of 7 and hydrolysis
time of 1 hr. It was found that the predicted value of ACE inhibitory activity (97.21%)
was close to that of experimental value (90.34%) with the IC50 of 0.425±0.05 mg/ml.
Amiza Mat Amin, Zaliha Harun, Intan Liyana Muhamad Roslan. (2019) Optimization of enzymatic protein hydrolysis conditions to obtain maximum angiotensin-I converting enzyme (ACE) inhibitory activity from flower crab (Portunis pelagicus) meat, Asian Journal of Agriculture and Biology, Volume 7, Issue 1.
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