Abstract
This paper will discuss, in vitro investigation of chemical constituents extracted from the stem
bark of Dipterocarpus verrucosus, Dipterocarpus crinitus and Dipterocarpus cornutus against
opportunistic filamentous fungi. In this research, 17 compounds comprised of twelve
oligostilbenoids, (-)--viniferin ,(-)-laevifonol, (-)-hopeaphenol , (-)-isohopeaphenol, vaticanol
B, diptoindonesin E, hemsleyanol D, davidiol A, resveratrol, ampelopsin A, ampelopsin F,
together with three other phenolic; gallic acid derivative, (-)-bergenin, scopoletin and 4-
methoxygallocathecin and also two terpene; β-sitosterol and β-sitosterol glucoside have been
isolated. In this study, the crude extracts and isolated compounds were evaluated regarding to
their antifungal activity; in terms of MIC, MFC and germination assay against pathogenic fungi
strains, namely Aspergillus flavus (AF), Aspergillus oligosporus (AO), Rhizophus oryzae (RO)
and Fusarium oxysporum (FO) using Clinical and Laboratory Standard Institute (CLSI)
methods. The MIC of crude extracts and isolated compounds against all fungi ranged from 3.8
- 500 µg/mL. F. oxysporum shows the most sensitive microorganisms on crude extract: D.
verrucosus, D. cornutus and isolated compound: ε-viniferin with MIC of 3.8 µg/mL. The MIC
was lower compared to amphotericin B (4 µg/ml). The strain was killed at the MFC of 31.3,
31.3 and 15.6 µg/mL respectively, as compared to amphotericin B (8 µg/mL). Compounds:
resveratrol, laevifonol, ε-viniferin, ampelopsin F, vaticanol B, vaticanol A, isomer of
hopeaphenol and isohopeaphenol, β-sitosterol and β-sitosterolglucoside possessed an inhibitory
activity on the conidial germination of F. oxysporum at the concentration of 4× MIC. On top of
that, D. cornutus, ampelopsin A and hemsleyanol D possessed a complete sterility at the
concentration of 2×MIC while D.verrucosus achieved its inhibitory activity at 1× MIC. To the
best of our study, there is no data discussing the inhibition of conidial germination of
filamentous fungi using the tested compounds and crudes tested.
Wan Mohd Zain, Norizan Ahmat, Yaya Rukayadi, Puteh Osman, Asma Husna, Neneng Winda. (2019) In vitro antimycotic activity of chemical constituents from Dipterocarpus verrucosus, Dipterocarpus cornutus and Dipterocarpus crinitus against opportunistic filamentous fungi, Asian Journal of Agriculture and Biology, Volume 7, Issue 3.
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