Abstract
The study on in-vitro fertility assessment of frozen thawed semen from Kundhi bulls was undertaken during the months of July to
September 2006 at the Department of Animal Reproduction, Faculty of Animal Husbandry and Veterinary Sciences, Sindh
Agriculture University Tandojam. Before freezing, each ejaculate was assessed for volume, color, mass activity, and motility and
sperm concentration. Twenty samples having motility 60% or above were frozen for post-thaw assessment. Frozen thawed semen was
incubated at 250C for 5 hours and examined for progressive linear motility and live dead sperm count. It was observed that all the
ejaculates were creamy white in color. The mean (± SEM) volume, mass activity, motility percentage sperm concentrations and pH of
the semen from Kundhi buffalo bulls were found to be 2.79 ± 0.217 ml, 2.85 ± 0.111, 71.75 ± 2.621%, 11.35 ± 1.255 millions/ml and
5.82 ± 0.092 respectively for fresh semen. No significant (P>0.05) difference was found between the parameters except for pH value,
which was significantly (P< 0.03) different between the bulls. The mean (± SEM) sperm motility percentage was found to be
20.46±1.62 and dead sperm count were 6.9± 0.2% for frozen semen of Kundhi buffalo bull. A significant (P< 0.05) difference was
found between the bulls for post-thaw motility percentage. It was found that at one hour of incubation period motile sperms were
43.25 ± 2.95% having 11.78 ± 0.28% dead sperm count. After 5 hours incubation all sperms were found dead. It was concluded that
sperms maintaining long term motility and having less dead sperm count are suitable for artificial insemination.
