Abstract
A variety of organisms were isolated from conjunctivas of 32 subjects, to evaluate the occurrence of different gram positive and gram
negative bacteria in normal and diseased subjects. The specimens were obtained from individuals belonging to 2-61 years of age
which were divided into 6 groups with respect to age. One hundred and six isolates were obtained from 64 specimens. Thirty eight
(36%) specimens revealed single isolates whereas double, triple and quadruple isolates were obtained from 30%, 13% and 8%
specimens, respectively. Culture results were negative for 13% specimens. Characterization of ocular flora isolates to genus level
revealed that Staphylococcus (64%) was the most frequent genus isolated from conjunctivas. Micrococcus (10%)turned out to be the
second most prevalent genus followed by Bacillus (7%), Streptococcus (5%), Corynebacterium (5%), Pseudomonas (3%), Moraxella
(2%), Neisseria (1%), Branhamella (1%), Acinetobacter (1%), Hemophilus (1%) and Klebsiella (1%). The incidence rate of overall
number of isolates with respect to sex was noted as 34/106 in males and 72/106 in females. The number of isolates obtained in
different age groups was 5, 10, 43, 8, 26 and 14 from age groups of 2-11, 12-21, 22-31, 32-41, 42-51 and 52-61 years, respectively
