Abstract
A total of 23 isolates of Trichoderma spp. collected from different areas of Pakistan, were procured from First Fungal Culture Bank of Pakistan (FCBP), Institute of Plant Pathology, University of the Punjab, Lahore. On the basis of morphological and cultural characteristics, Trichoderma isolates were identified into four species, namely T. harzianum, T. hamatum, T. koningii and T. pseudokoningii. The genomic homology of these isolates of Trichoderma was examined using RAPD analysis. Out of 27 random decamer primers, five primers viz., A-11, A-12, A13, B05 and B-18 were screened out at 25oC optimized Tm (melting temperature). The amplification of bands were observed in the range of 250bp50000bp by all five primers. In cluster analysis of five primers, isolate of T. koningii (wood of M. indica) showed least genomic similarity of 47.30% in comparison to all others. The other isolates of T. harzianum, T. hamatum, T. koningii and T. pseudokoningii showed maximum genomic homology (~78%). This data will be useful to achieve authentic identification for maximum usage of these microbes in all research fields.
