Studies on tripartite interactions between vesicular arbuscular mycorrhiza—VAM, root lesion nematode and wheat varieties were carried out in soil pots to find out the potentiality of VAM fungus (Glomus mosseae) in limiting the activities of root lesion nematode (Pratylenehus sp.) in rhizospheres of 6 wheat varieties, while resistance / susceptibility of the wheat varieties towards the nematode / VAM fungus was also observed. On single (separate) inoculations (G. mosseae 1 Fratylenchus sp.) Blue silver showed positive response towards VAM fungus (85.58 ± 4.7 % infection) but least to nematode in term of diseases severity—DS (16.75 ± 6.8 %). However, the var. Mexi Pak showed susceptibility towards nematode (48.08 ± 08 % DS) and comparatively less response towards VAM fungus (55.18 + 7.7 % infection). The combined inoculations (G. rnosseae Pratylenchus sp.) showed significant reduction (P<0.05) in root DS and the population of Pratylenchus sp. in rhizospheric regions of all wheat varieties except Mexi Pak as compared to control set of pots. Blue Silver showed maximum reduction in the population of Pratylenchus sp. (62.5 ± 04.71 %) while it was least in Mexi Pak (06.8 ± 05.56 %).