Four cotton cultivars were studied at seedling stage in salinized nutrient growth medium having 0, 75,150 and 250 mol m-3 NaCI salinity. After salinization, the seedlings were harvested after day 3 and the 2nd batch after 17 day. The plant parts were analyzed for Na+ contents. The tolerant cultivar NIAB 78 accumulated significantly higher Na+ concentration in stem than the sensitive D 9 and Ravi. NIAB 78 maintained lower Na+ concentration in leaf than the sensitive ones. The reason of tolerance in NIAB 78 may be the maintenance of lower Na+ accumulation in leaves than stem. Sodium transport (JNa + ) of NIAB 78 was higher than D 9 and Ravi. Such a trend was related to the demand for solutes set up by the growing plants for osmoregulation.