Abstract
The mechanism of loss of viability and vigour in seeds is by no means resolved. In this study seeds were exposed
to high temperature and high humidity, a technique widely used to study seed storability and deterioration. The
effect of environmental treatments on seed germ inability, seedling vigour, electrolyte leakage and chromosomal
aberration in pea seeds was compared. Ageing treatment showed rapid and significant reductions in rate of seed
germination and seedling vigour. Loss of viability and declining vigour were associated with the increased leakage
and chromosomal aberrations. The common effect of seed ageing on the seeds integrity is described as function
of free radical mediated membrane and chromosomal damage.
Key Words: Peas, vigour, leakage, ageing, chromosomal damage
