Abstract
Reintroduction of calcium into calcium depleted rat heart, so called Ca-paradox (CP), results in extensive biochemical, physiological and histological changes. Free radical producing enzyme xanthine oxidase (XO) was localized (in situ) in PLP fixed thick frozen sections of normal and Ca-paradox rat hearts by enzyme histochemistry using nitroblue tetrazolium (NBT) staining and also by immunocytochemistry using murine monoclonal antibodies against XO. An immunoperoxidase reaction product was accumulated in interstitial cells, endothelial cells of small capillaries and blood vessels in normal rat heart whereas it was markedly increased in Ca-paradox tissues, but XO activity was reduced by nitroblue tetrazolium staining which suggested the loss of enzyme activity. The results indicate that the XO activity during Ca-paradox conditions is increased and is restricted to interstitial and endothelial cells and its activity in sarcolemma membranes is due to rupturing and shedding of the cytoplasmic materials of interstitial and endothelial cells. The activity of XO along with CP may enhance the myocardial abnormalities.
ZAHOOR QADIR SAMRA, ZAHIDA SIDDIQUE . (1998) DISTRIBUTION OF XANTHINE OXIDASE DURING CALCIUM PARADOX CONDITIONS OF RAT HEART , Punjab University Journal of Zoology, Volume 13, Issue 1.
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