Abstract
Background: Tobacco Smoking, most commonly,can cause the diseases affecting thelungs and heart. Human gut microbiota plays a key role to decide the health status of the host. Current studyaimed to characterize the gut microbiota of healthyChinese tobacco smokers and to study the alteration in diversity and similarity of gut microbiota, with comparison of healthy non-smokers.Methods: Fecal samples were collected from fourteen healthy tobacco smokers and six fromhealthy non-smoker individuals. PCR-denaturing gradient gel electrophoresis, with universal primers focusing V3 region of the 16S rRNA gene, wasdone to characterize the overall gut microbial composition of healthy tobacco smokers in comparison with healthy non-smoker subjects and some strongly dominant gel bands were excised for sequencing. Real time PCR wasalsoperformed to evaluate the copy numbers of some dominant bacteria of intestinal flora. Results: The results indicated that gut microbial diversity intobacco smoker group was lower than non-smoker controls. Furthermore, similarity index comparison also indicated that it was lower in inter-group than intra-group, which showed that gut microbial composition was changed in tobacco smoker group. Sequencing results also indicated a change in bacterial composition between both groups. We also observed that in tobacco smoker group, there was a significant reduction in Bifidobacterium and non-significant increase in Bacteroides vulgatus, while non-significant decrease in Lactobacillus andclostridium leptum sub group, respectively. Conclusion: It can be concluded that in healthy Chinesetobacco smoker group, there is a notable alteration inthe molecular characterization of gut microbiota
Prof. Dr. Jiru Xu,, Muhammad Shahzad, Xiaokang , , Chaofeng Ma, ,Jiru Xu. (2017) MOLECULAR CHARACTERIZATION OF FECAL MICROBIOTA OF HEALTHY CHINESE TOBACCO SMOKER SUBJECTS IN SHAANXI PROVINCE, XI’AN CHINA, JOURNAL OF AYUB MEDICAL COLLEGE ABBOTTABAD, Volume 29, Issue 1.
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