Abstract
Objective: To determine the frequency of bacteria
colonizing central venous catheter line and their
antimicrobial susceptibility pattern.
Methodology: This cross sectional study was
conducted in the department of pathology, Sahara
Medical College, Narowal from January to June
20201. Sample size was calculated using WHO sample
size calculator and we used non-probability
consecutive sampling technique.Central venous
catheter tip colonization was defined as bacterial
colony count ≥15 CFU/ml by semi quantitative
technique. Antibiotic susceptibility pattern of isolated
bacteria was determined using Disc Diffusion Method
as recommended by CLSI guidelines. Data were
analyzed using SPSS version 17.
Results: A total 125 specimens were obtained from
125 patients. Micro-organisms growth was obtained on
110(88%) specimens. Gram-positive bacteria were
isolated in 60(54.5%) specimens, gram-negative
bacteria in 23(20.9%) specimens. Vancomycin and
linezolid were most effective against gram positive
bacteria while colistin and imipenem against gram
negative organisms.
Conclusion: Bacteria colonizing central venous
catheter line were very common. Most organisms were
gram positive and sensitive to vancomycin and
linezolid.
Keywords: Antimicrobial susceptibility, isolated
bacteria, central venous catheter.
