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In herbivore insects, RNAi can be achieved by feeding target insects on plants produce double standard RNA (dsRNAs) either by stable or transient transformation. Production of dsRNAs through stable transformation is a slow and laborious process, so, here we demonstrated a quick and efficient transient method. We used Potato Virus X (PVX) as a viral vector to produce dsRNAs transiently in the host plant Nicotiana tabacum which cause quick gene knockdown of target gene (chitin synthase) in herbivore insect, mealybug (Phenacoccus solenopsis). Reverse-transcriptasepolymerase chain reaction RT-PCR validate the expression of transgene in recombinant-PVX-inoculated plants, whereas, population of mealybug was significantly reduced (30%) just after 48hrs of feeding on recombinant PVX infected plants and reached upto 78% in 7-9days. The quick knockdown of target gene and mortality of the insects feeding on recombinant PVX infected plant validate that the recombinant PVX is an effective tool for evaluating candidate RNAi effectors in plants.
Imran Rauf, Raza Muhammad Memon, Noroza Umar, Rubab Zahra Naqvi, Imran Amin, Shahid Mansoor. (2021) TRANSIENT POST-TRANSCRIPTIONAL GENE SILENCING IN NICOTIANA TABACUM FOR QUICK ASSESSMENT OF TARGET GENE EFFICACY, , Volume 18, Issue 4.
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