• Farrukh Abu Hazim

• Syeda Mariam Siddiqa

• Muhammad Mumtaz Khan

• Mehwish Kalam

• Rizwana Yasmin

• Mustafa Kamal

تلخیص

The present investigation targets the study of structural changes in the genome of Hepatitis C Virus 3a, secondary to mutations in interferon sensitivity determining region (ISDR) of NS5A and subsequent assessment of possible outcomes. We included five hundred patients in our study. We analyzed mutations in interferon sensitivity determining region (ISDR) of NS5A. The study was done as part of Ph.D. research project in the Department of Biotechnology, University of Karachi, where nested PCR of HCV and Gel electrophoresis of isolates were performed. Other relevant lab parameters were carried out in Rahila Diagnostic Research and Reference Lab. (Pvt) Ltd; including qualitative & quantitative RT-PCR and genotyping. The most notable substitutions found in this region were C2221T, R2225G, H2227N, A2230V and V2233M. We evaluated these mutational changes in ISDR through structural modeling. The differences in size, polarity and hydrophobicity of mutant amino acid residues from wild type reflect marked phenotypic changes. This study provides better understanding of effects of genomic changes that would be helpful to adopt modified approaches in the treatment and prevention of this morbid viral infection

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