Abstract
Objective: To determine the hepatoprotective effect of Aqueous extract of Stem Bark of Berberis Lycium Royale
in Isoniazid (INH) induced hepatotoxicity in mice.
Study Design: Experimental study.
th th Place and Duration of Study: The study was conducted from 7 April 2014 till 7 May 2014 at animal house of
National Institute of Health, Islamabad.
Materials and Methods: Fifty six male albino Balb/C mice were randomly divided into four groups i.e Group I:
Control group (C), Group II: Drug treated group (D), Group III: Low Aqueous group (LA), Group IV: High Aqueous
group (HA) of 14 mice each. INH (50 mg/kg) was given to induce hepatotoxicity in all groups except control
group (C). Aqueous extract of stem bark of Berberis Lycium Royle was given in low doses (150 mg/kg) to Low
Aqueous group (LA) and in high doses (200 mg/kg) to High Aqueous group (HA). Blood samples were collected
th th
at day (0, 15 & 30 ) for estimation of Liver function tests (LFTs). At the end samples for liver histopathology
were taken.
Results: INH produced severe hepatotoxicity as depicted by raised LFT's & severe steatosis, hepatocytic
ballooning & inflammation. In animals of Low Aqueous group (LA) and High Aqueous group (HA) , serum levels
of biomarkers were decreased and their liver sections showed improved histological picture but the reduction
in toxic effects were more pronounced in animals treated with high Aqueous group. (HA).
Conclusion: Aqueous extract of stem bark of Berberis Lycium Royale has more significant hepatoprotective
potential in high doses as compare to low doses.
Saima Rafiq, Adnan Jehangir, Samina Iltaf. (2015) Hepatoprotective Effect of Aqueous Extract of Stem Bark of Berberis Lycium Royale in Isoniazid Induced Hepatotoxicity in Mice, Journal of Islamic International Medical College, Volume-10, Issue-4.
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